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. Author manuscript; available in PMC: 2023 Nov 18.
Published in final edited form as: J Vis Exp. 2022 Sep 13;(187):10.3791/64176. doi: 10.3791/64176
Name of Material/ Equipment Company Catalog Number Comments/Description
Bafilomycin A1 Research Products International B40500
Bleomycin sulfate Cayman 13877
Bovine serum albumin (BSA) US Biological A1380
Calcein Violet 450 AM viability dye ThermoFisher Scientific 65–0854-39 eBioscience
DPP4 antibody, PE conjugate Biolegend 137803 Clone H194–112
Cell line: A549 human lung adenocarcinoma American Type Culture Collection CCL-185
Cell line: B16-F10 mouse melanoma American Type Culture Collection CRL-6475
Cell scraper Corning 3008
Cell strainers, 100 μm Falcon 352360
DDAO-Galactoside Life Technologies D6488
DMEM medium 1X Life Technologies 11960–069
DMSO Sigma D2438
DNAse I Sigma DN25
Doxorubicin, hydrochloride injection (USP) Pfizer NDC 0069–3032-20
Doxorubicin, PEGylated liposomal (USP) Sun Pharmaceutical NDC 47335–049-40
EDTA 0.5M Life Technologies 15575–038
Etoposide Cayman 12092
FBS Omega FB-11
Fc receptor blocking reagent Biolegend 101320 Anti-mouse CD16/32
Flow cytometer (cell analyzer) Becton Dickinson (BD) Various LSRFortessa
Flow cytometer (cell sorter) Becton Dickinson (BD) Various FACSAria
GlutaMax 100X Life Technologies 35050061
HEPES 1M Lonza BW17737
Liberase TL Sigma 5401020001 Roche
Paraformaldehyde 16% Electron Microscopy Sciences 15710
Penicillin/Streptomycin 100X Life Technologies 15140122
Phosphate buffered saline (PBS) 1X Corning MT21031CV Dulbecco’s PBS (without calcium and magnesium)
Rainbow calibration particles, ultra kit SpheroTech UCRP-38–2K 3.5 – 3.9 um, 2E6/mL
RPMI-1640 medium 1X Life Technologies 11875–119
Sodium chloride 0.9% (USP) Baxter Healthcare Corporation 2B1324
Software for cytometer data acquisition, “FACSDiva” Becton Dickinson (BD) n/a Contact BD for license
Software for cytometer data analysis, “FlowJo” TreeStar n/a Contact TreeStar for license
Trypsin-EDTA 0.25% Life Technologies 25200–114