Figure 4.

RT-PCR analysis of pre-mRNA and mRNA levels during L4440-induced silencing of elt-2::gfp/LacZ. (A) RT-PCR of gfp/LacZ transgene; amplification with exon-specific primers (top) detects products from the mature mRNA (357 bp) and pre-mRNA (458 bp); amplification with intron-specific primers (middle) detects a product of 373-bp; ama-1 mRNA RT-PCR detecting a 236-bp product is shown as control (bottom). (B) Quantification of the relative levels of gfp/LacZ mRNA and gfp/LacZ pre-mRNA shown in A by real-time RT-PCR; ama-1 expression was used for internal reference. Mean values and ranges of the relative LacZ/ama-1 ratios based on two real-time RT-PCR trials are shown.