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. 2005 Mar 15;19(6):683–696. doi: 10.1101/gad.1247705

Figure 6.

Figure 6.

elt-2::gfp/LacZ silencing is associated with decrease in histone H4 acetylation and polymerase II association. (A) ChIP with anti-acetyl-H4 antibodies was performed on extracts from rrf-3; elt-2::gfp strain with (right) and without (left) L4440 feeding. PCR products were amplified with primers specific for the gfp/LacZ transgene (top), 2-kb backbone sequence (bottom) or act-3 gene (middle) as an internal control. (Lane 1) DNA purified from 1/40 of input material was used. (Lane 2) DNA bound to agarose A beads with anti-acetyl-H4 antibodies. (Lane 3) DNA bound to agarose A beads without antibody. (B,C) Real-time PCR quantification of the relative amounts of gfp/LacZ DNA precipitated by anti-acetyl-H4 antibodies or 8WG16 Pol II antibodies at indicated conditions, act-3 was used as internal reference. Mean values and ranges of the relative LacZ/actin ratios from two to three independent experiments are shown.