Table 3.
Genes affecting RNAi-TGS of elt-2::gfp/LacZ
| Genes involved in RNAi
| ||||||
|---|---|---|---|---|---|---|
| Gene | Name | Domain/function | Desilencing in WT | Desilencing in rrf-3 mutant | ||
| B0379.3 | mut-16 | Chromatin organization (Vastenhouw et al. 2003) | ++ | ++ | ||
| W02D3.8 | smg-5 | Nucleotide binding protein (Domeier et al. 2000) | ++ | −− | ||
| F16D3.2 | rsd-6 | Tudor domain (Tijsterman et al. 2004) | ++ | ++ | ||
| F10G7.2 | tsn-1 | Tudor domain (Caudy et al. 2003) dsRNa binding, RNAse III | ++ | −− | ||
| K12H4.8 | dcr-1 | (Grishok et al. 2001; Knight and Bass 2001) | ++ | ++ | ||
| C34E11.1 | rsd-3 | ENTH domain (Tijsterman et al. 2004) | ++ | −− | ||
| K08H10.7 | rde-1 (ne300) | PAZ/PIWI domain (Tabara et al. 1999) | +++ | ND | ||
| T20G5.11 | rde-4 (ne299) | dsRNA binding (Tabara et al. 2002) | +++ | ND | ||
| F26A3.8 | rrf-1 (pk1417) | RdRP (Sijen et al. 2001) | +++ | ND | ||
| Genes implicated in transposon silencing (Vastenhouw et al. 2003) | ||||||
| K07A12.3 | asg-1 | mitochondrial ATP synthase g subunit | ++ | −− | ||
| F55F8.3 | G-protein beta WD-40 repeat, similar to U4/U6 SNP | ++ | ++ | |||
| W01B11.3 | snoRNA binding | ++ | ++ | |||
| ZK858.7 | Translation initiation factor | ++ | −− | |||
| Y54E5A.4 | npp-4 | Nucleoporin FG repeat | ++ | + | ||
| Y106G6H.2 | pab-1 | Poly(A) binding protein | ++ | −− | ||
| F35G12.10 | asb-1 | Mitochondrial ATP synthase | ++ | −− | ||
| M01F1.3 | Lipoic acid synthase, radical SAM | ++ | ++ | |||
| F37C12.4 | rpl-36 | Ribosomal protein L36 | ++ | ++ | ||
| C08F8.2 | ATP dependent helicase activity | ++ | −− | |||
| D2096.8 | Nucleosome assembly protein | ++ | −− | |||
| Genes with a chromatin-related function | ||||||
| C08B11.2 | hda-2 | Histone deacetylase (Pothof et al. 2003) | ++ | −− | ||
| C04A2.3 | egl-27 | BAH/ELM2 domain (Solari et al. 1999) | ++ | −− | ||
| T09A5.8 | Chromo domain | ++ | −− | |||
| K01G5.2 | hpl-2 | Chromo domain (Couteau et al. 2002) | ++ | −− | ||
| T26A5.7 | set-1 | SET domain (Terranova et al. 2002) | + | −− | ||
| C26E6.9 | set-2 | SET domain (Terranova et al. 2002) | ++ | −− | ||
| T21B10.5 | Contains Zn-finger, SET domains | + | −− | |||
| Y43C5A.6 | rad-51 | DNA binding (Takanami et al. 1998) | ++ | −− | ||
| M04B2.3 | gfl-1 | Chromatin binding (Dudley et al. 2002) | ++ | −− | ||
| K08H2.6 | hpl-1 | Chromo domain (Couteau et al. 2002) | + | −− | ||
| Y37D8A.9 | mrg-1 | Chromo domain (Fujita et al. 2002) | ++ | ++ | ||
| F54F2.2 | zfp-1 | Chromatin/DNA binding (Dudley et al. 2002) | ++ | −− | ||
| C50E10.4 | sop-2 | SAM domain, Hox gene regulation (Zhang et al. 2004a,b) | ++ | + | ||
| C25A1.10 | dao-5 | Nuclear phosphoprotein, similar to sop-2 (BLAST) | ++ | −− | ||
| F55F8.4 | Related to Tas3 (Verdel et al. 2004) | + | −− | |||
| Genes involved in RNA binding and/or translational regulation | ||||||
| F18A11.1 | puf-6 | Pumilio/Puf RNA binding (Wickens et al. 2002) | ++ | −− | ||
| B0273.2 | puf-7 | Pumilio/Puf RNA binding (Wickens et al. 2002) | ++ | −− | ||
| F48F7.1 | alg-1 | PAZ/PIWI domain (Grishok et al. 2001) | + | −− | ||
| B0035.12 | RNA binding region, poly(A) binding | ++ | ++ | |||
| D1037.1 | dsRNA binding | ++ | −− | |||
| Genes involved in sumoylation | ||||||
| K12C11.2 | smo-1 | Ubiquitin-like protein | + | −− | ||
| W02A11.4 | uba-2 | SUMO-activating complex | ++ | + | ||
(+++) 100% of worms desilenced; (−) 80-100% of worms completely silenced; (++) 80-100% of worms desilenced; (−−) 100% of worms completely silenced; (+) >50% of worms desilenced.