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. 2005 Mar 15;19(6):733–743. doi: 10.1101/gad.326105

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Copyright © 2005, Cold Spring Harbor Laboratory Press

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Figure 1. — Olig3 marks a dorsal progenitor domain in the spinal cord. Immunofluorescence analysis of the developing mouse spinal cord using antibodies directed against Olig3 (green) at E10.5 (A,C-F) and E12.5 (B). In addition, Mash1- (A,B,F), Math1- (C), Ngn1- (D), and Ngn2- (E) expressing cells were visualized (red, the overlap with green appears yellow). Three distinct Olig3⁺ progenitor domains (d1-d3) can be distinguished, which contain cells that coexpress Olig3 and either Math1 (d1), Ngn1 (d2), or Mash1 (d3). Ngn2 is present in cells of the d2 and d3 domains, and Mash1 is in d3 and in ventrally abutting progenitors (see also Fig. 3C for a summary). Bars, 100 μm.