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. 2023 Nov 18;9:419. doi: 10.1038/s41420-023-01712-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Experimental timeline of D-gal and DFO treatment in C57 mice. B SA-β-Gal staining of mouse retina. Scale bars, 100 μm. C The fundus photograph of mice. Scale bars, 500 μm. D The outer nuclear layer (ONL) area in horizontal optical coherence tomography scans (yellow line) was quantified by ImageJ (n = 3). E The H&E staining of mouse retina (n = 3). Scale bars, 100 μm. F Immunohistochemistry analysis of 8-OhdG, 4-HNE and MDA in the mouse retina. Scale bars, 100 μm. G Arrestin, Rhodopsin and p-H2AX in the mouse retina was evaluated by immunofluorescence. Scale bars, 25 μm. H The protein content of NCOA4, Ferritin, LC3B, cGAS, p-STING and STING in the mouse retina was assessed by Western blot (n = 3). Data are shown as Mean ± SD. *p < 0.05 vs. control group. ^#p < 0.05 vs. D-gal group.