Fig. 3. Single-cell transcriptional landscape in TAK-931-treated aneuploid cells.

a tSNE plot based on the gene expression of DMSO- or TAK-931-treated HeLa cells. Clusters of 72 h DMSO-treated (blue) and TAK-931-treated (red) HeLa cells are shown. b Enrichment of inflammatory response hallmark in tSNE plots. Enrichment scores of the hallmark are colored yellow to orange. c Inflammatory gene expression in tSNE plots. Cells expressing CXCL8 (left) and TNFRSF1B (right) are colored in black (>1tag). d Dot plots of expression levels of CXCL8 (left) and TNFRSF1B (right) in single cells administered DMSO (blue) or TAK-931 (red). Plots are two independent experiments. Two-sided Welch Two Sample t test p < 0.000, p < 0.000, respectively. e Circle plots of the expressions of inflammatory-related genes CXCL1, CXCL8, IL1B, TNFSF15, and TNFRSF1B in DMSO- or TAK-931-treated cells. The circle color and size indicate average expression and expression percentage, respectively. f Heatmap of the enrichment scores, based on ssGSEA, between DMSO-treated (left) and TAK-931-treated (right) cells. Red indicates inflammatory-related hallmarks. g Violin boxplot of enrichment scores of inflammatory response, TNFA signaling pathway via NFkB, IL6-JAK-STAT3 signaling, IL2-STAT5 signaling, Notch signaling, and mTORC1 signaling in DMSO-treated (blue) and TAK-931-treated (red) cells. Plots are two independent experiments. All violin boxplots are defined by center lines (medians), box limits (25th and 75th percentiles), and whiskers (minima and maxima; the smallest and largest data range). Two-sided Welch Two Sample t test p < 0.000, respectively. h Comparison of enrichment scores between TNFA signaling and inflammatory response (left), between IL2-STAT5 signaling and TNFA signaling (right). DMSO-treated and TAK-931-treated cells are shown in blue and red, respectively. Source data are provided as a Source Data file.