Table 1.
Influence of different SIMSEF parameters on the scheduling of TIMS-MS2 precursors
| Method | Purity score | Quadrupole switch time / ms | Minimum distance of MS2 pixels/pixel | MS2 pixels | Precursor | MS2 events | Spectra coverage (SC) | Precursors/spot (avg/max) |
|---|---|---|---|---|---|---|---|---|
| 1 | 0.8 | 1.65 | 20 | 6477 | 1396 | 28,916 | 69.0% | 4.5/13 |
| 2 | 0.8 | 1.00 | 20 | 6224 | 1396 | 29,205 | 69.7% | 4.7/16 |
| 3 | 0.8 | 1.65 | 5 | 6508 | 1397 | 29,704 | 70.9% | 4.6/14 |
| 4 | 0.6 | 1.65 | 20 | 6996 | 1503 | 33,528 | 74.4% | 4.8/13 |
| 5 | 0.6 | 1.00 | 5 | 6725 | 1503 | 34,895 | 77.4% | 5.2/17 |
Changed parameters are printed in bold font. The TIMS-MS1 imaging dataset of sample R2c_1a (ramp time 150 ms, MS1 pixels 16,161, scheduling for 6 different collision energies with a replicate number of 5 MS2) was used. A total of 1514 precursor ions had at least one signal above an intensity of 5000 and were thereby eligible for scheduling. TIMS-MS2 scans were only scheduled if the intensity in a pixel exceeded 3000 and 20% of the maximum intensity. The final number of scheduled precursors is lower due to constraints on isolation purity, spatial restrictions, and quadrupole switch time.