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. 2023 Nov 18;14:7500. doi: 10.1038/s41467-023-43030-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — a Different stages of cytosolic lipid droplet formation from stage 1 to a mature stage 5 filled with astaxanthin. Details of astaxanthin-rich liquid subdomains are only visible in cryo-fixed cells. Stages are defined based on their size, connection with the endoplasmic reticulum, shown in purple, and mitochondria, in yellow, and density of astaxanthin domains. Mature LDs are disconnected from mitochondria, suggesting that the TAG they contain is not metabolically available for β-oxidation, and may therefore be stored for long periods. This observation has been made on 2 other cells in the FIB-SEM stack of cryo-fixed S. nivaloides cysts with similar result. b Interaction of lipid droplets with the endoplasmic reticulum and mitochondria. Mit mitochondria, ER endoplasmic reticulum, LD lipid droplet.