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. 2023 Nov 18;14:7515. doi: 10.1038/s41467-023-43280-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Schematics of chimeric proteins with swaps between PsHv1 and AtHv1 (a), and sequence details (b). c Representative proton currents elicited by membrane depolarization for the indicated chimeras and mutants, before and after mechanical stimulus (ΔP). Stimulation protocol as in Fig. 4b. d Averaged increases in current caused by the mechanical stimulus. I_A and I_B measured as in Fig. 4c. Bars are means of I_B/I_A from n biologically independent measurements ± SEM, n = 9 (PsHv1), 10 (AtHv1), 8 (ChE1-2), 6 (CHI2-3a), 7 (CHI2-3b), 10 (ChNC), 10 (ChE3-4.S4), and 11 (ChE3-4.S4.K). One-way Welch’s ANOVA with Dunnet’s T3 test was used for multiple comparison analysis, using PsHv1 as reference. p values were: 0.0030 (1), 0.0008 (2), 0.0003 (3), 0.9363 (4), 0.0093 (5), 0.4273 (6) and 0.0026 (7). Expression of ChE3-4.S4 did not result in measurable currents (n.c.). The I_B/I_A ratio for this chimera reflects the mechanosensitivity of the background leak current. Source data are provided as a Source Data file.