FIG. 1.

Dependence of forward light scatter intensity on particle characteristics. (A) Intensities calculated from Mie theory for three sizes of spheres as a function of m. Ordinate scales increase with particle size. (B) Intensities for latex microspheres of various sizes as predicted by Mie theory when m is 1.04 (—··—) or 1.19 (······) and by Rayleigh-Gans (37) theory (——). Data are measured intensities for microspheres with volumes and indicated standard deviations (error bars) computed according to diameters from electron microscopy reported by the supplier (•) and volumes determined with a Coulter Counter (○). The inset extends the particle size range. (C) Effect of axial ratio on forward light scatter intensity over a range of cell volumes as determined by Rayleigh-Gans theory. I_n/I₁ compares the intensity of a particle with an axial ratio of n to that of a sphere of the same volume.