TABLE 5.
Culture | Subpopulationb | Population (106 cells/ ml) | Cell mass (pg/cell)
|
Biomass (μg/liter)
|
DNA content (fg/cell) | ||
---|---|---|---|---|---|---|---|
Dryc | Wetd | Drye | Wetf | ||||
C. oligotrophus | Total | 0.484 | 0.042 | 0.234 | 20.2 | 113.2 | 5.0 |
1 n | 0.191 | 0.027 | 0.151 | 5.2 | 28.8 | 3.2 | |
2 n | 0.173 | 0.040 | 0.223 | 7.0 | 38.6 | 5.8 | |
3 n | 0.015 | 0.073 | 0.408 | 1.1 | 6.1 | 8.9 | |
C phase | 0.074 | 0.032 | 0.179 | 2.4 | 13.2 | 4.2 | |
Marinobacter sp. strain T2 | Total | 0.434 | 0.172 | 0.896 | 74.6 | 388.8 | 4.1 |
E. coli | Total | 0.492 | 0.497 | 1.74 | 244.5 | 856.1 | 8.7 |
1 n | 0.117 | 0.368 | 1.29 | 43.1 | 150.9 | 5.4 | |
2 n | 0.263 | 0.488 | 1.71 | 128.3 | 449.7 | 10.2 | |
3 n | 0.010 | 0.688 | 2.41 | 6.9 | 24.1 | 14.9 | |
4 n | 0.014 | 0.811 | 2.84 | 11.4 | 39.8 | 20.6 | |
Dim | 0.048 | 0.500 | 1.75 | 24.0 | 84.0 | 1.7 |
Mixture of C. oligotrophus, Marinobacter sp. strain T2, and E. coli, as shown in Fig. 4.
Total includes all cells from a region; n indicates the number of chromosomes per cell; C phase indicates cells undergoing chromosome replication; Dim indicates cells with very low fluorescence. The sum of values for the subpopulations is >91% the total for all organisms in regions electronically bounded as shown.
From flow cytometry as shown in Fig. 2, with corrections for E. coli in M9 medium.
Dry mass divided by (Xdry/Xwet) (Table 4; 17.9% for C. oligotrophus).
Population density × dry mass per cell.
Population density × wet mass per cell.