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. 1998 Oct;64(10):3900–3909. doi: 10.1128/aem.64.10.3900-3909.1998

TABLE 5.

Subpopulations in a mixture of three bacterial cultures according to biomass and DNA contenta

Culture Subpopulationb Population (106 cells/ ml) Cell mass (pg/cell)
Biomass (μg/liter)
DNA content (fg/cell)
Dryc Wetd Drye Wetf
C. oligotrophus Total 0.484 0.042 0.234 20.2 113.2 5.0
1 n 0.191 0.027 0.151 5.2 28.8 3.2
2 n 0.173 0.040 0.223 7.0 38.6 5.8
3 n 0.015 0.073 0.408 1.1 6.1 8.9
C phase 0.074 0.032 0.179 2.4 13.2 4.2
Marinobacter sp. strain T2 Total 0.434 0.172 0.896 74.6 388.8 4.1
E. coli Total 0.492 0.497 1.74 244.5 856.1 8.7
1 n 0.117 0.368 1.29 43.1 150.9 5.4
2 n 0.263 0.488 1.71 128.3 449.7 10.2
3 n 0.010 0.688 2.41 6.9 24.1 14.9
4 n 0.014 0.811 2.84 11.4 39.8 20.6
Dim 0.048 0.500 1.75 24.0 84.0 1.7
a

Mixture of C. oligotrophus, Marinobacter sp. strain T2, and E. coli, as shown in Fig. 4

b

Total includes all cells from a region; n indicates the number of chromosomes per cell; C phase indicates cells undergoing chromosome replication; Dim indicates cells with very low fluorescence. The sum of values for the subpopulations is >91% the total for all organisms in regions electronically bounded as shown. 

c

From flow cytometry as shown in Fig. 2, with corrections for E. coli in M9 medium. 

d

Dry mass divided by (Xdry/Xwet) (Table 4; 17.9% for C. oligotrophus). 

e

Population density × dry mass per cell. 

f

Population density × wet mass per cell.