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. Author manuscript; available in PMC: 2023 Nov 19.
Published in final edited form as: Nature. 2023 May 10;617(7962):785–791. doi: 10.1038/s41586-023-06053-0

Extended Data Fig. 7: In-situ hybridization corroborating evidence for marker localization in single cell/nuclei RNA-seq profiles in maize.

Extended Data Fig. 7:

a-n in situ hybridization using Hairpin Chain Reaction (HCR) probes labeling various transcripts. Cross sections are on the left and longitudinal sections are on the right. UMAPs showing each transcript’s cluster localization are displayed next to each probe’s fluorescent image. Additionally, spatial transcriptomics imaging data of the same probe is shown in the right column for (c-e). The minimum/maximum values for each fluorescence channel (grey: autofluorescence, magenta: HCR probes) have been adjusted to show the localization more clearly in the merged image.