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. Author manuscript; available in PMC: 2023 Nov 19.
Published in final edited form as: Nature. 2023 May 10;617(7962):785–791. doi: 10.1038/s41586-023-06053-0

Extended Data Fig. 8: In-situ hybridization corroborates evidence for localization of marker gene expression from single-cell RNA-seq profiles in sorghum.

Extended Data Fig. 8:

a-i In situ hybridization using Hairpin Chain Reaction (HCR) probes labeling various transcripts. Cross sections are on the left and longitudinal sections on the right (a,c,d,e). Longitudinal sections are shown in (f,g,h,i). UMAPs showing each transcript’s cluster localization are shown next to each probe’s fluorescent image. The minimum/maximum values for each fluorescence channel (grey: autofluorescence, magenta: HCR probes) have been adjusted to show the localization more clearly in the merged image.