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. 1998 Oct;64(10):3910–3916. doi: 10.1128/aem.64.10.3910-3916.1998

FIG. 2.

FIG. 2

Western blot of 15 μg of protein from FWC or inclusion bodies of recombinant and wild-type B. sphaericus strains and of 5 μg of purified Cyt1Ab1 inclusion bodies from B. thuringiensis SPL407(pcyt1Ab1). The filter was incubated with antiserum (dilution 1/2,000) raised against Cyt1Ab1 protein purified from B. thuringiensis SPL407(pcyt1Ab1). Lanes: 1, 2297(pcyt1Ab1); 2, wild-type strain 2297; 3, nonsporulating mutant 2297(pcyt1Ab1); 4, nonsporulating mutant 2297(pMK3); 5, purified inclusion bodies of 2297(pcyt1Ab1); 6 and 7, purified inclusion bodies of nonsporulating mutant 2297(pcyt1Ab1) with 15 and 30 μg of protein, respectively; 8, Iab872(pcyt1Ab1); 9, 2362(pMK3); 10, 2362(pcyt1Ab1); 11, purified Cyt1Ab1 inclusion bodies; 12, wild-type strain Iab872; 13, Iab872(pMK3); 14, Iab872(pcyt1Ab1).