FIG. 3.

Protein analysis after sonication of FWC, inclusion body, and spore-pellet (after ultracentrifugation) suspensions from wild-type and recombinant B. sphaericus 2297 strains. Fifteen micrograms of protein per well was subjected to SDS-PAGE (12% polyacrylamide). Lanes: 1 to 3, wild-type strain 2297 (lane 1, FWC; lane 2, inclusion body suspension; lane 3, spore pellet); 4 and 5, mutant nonsporulating strain 2297(pMK3) (lane 4, FWC; lane 5, inclusion body suspension); 6 to 8, recombinant 2297(pcyt1Ab1) (lane 6, FWC; lane 7, inclusion body suspension; lane 8, spore pellet); 9 and 10, mutant nonsporulating strain 2297(pcyt1Ab1) (lane 9, FWC; lane 10, inclusion body suspension); MW, standard protein markers from Pharmacia low-molecular-mass kit.