TABLE 2.
Larvicidal activity of B. sphaericus recombinant strains against C. pipiens subsp. pipiens (SPHAE) and C. quinquefasciatus (GeoR) larval populations resistant to B. sphaericus
| Mosquito strain | Strain (FWC)a | Larvicidal activity (μg/ml)
|
|
|---|---|---|---|
| LC50b | LC90 | ||
| C. quinquefasciatus | 2297 (parental) | 134.0 ± 6.7c | 383.6 ± 28.4 |
| 2297(pcyt1Ab1) | 6.0 ± 2.8 | 16.4 ± 8.8 | |
| C. pipiens subsp. pipiens | 2297 (parental) | 193.2 ± 46 | 611.4 ± 42 |
| 2297(pcyt1Ab1) | 14.1 ± 7.4 | 38.8 ± 18 | |
| 2362 (parental) | 66.8 ± 24.9 | 115.2 ± 65 | |
| 2362(pcyt1Ab1) | 45.8 ± 7.8 | 122.3 ± 67 | |
| 2297 asporulated(pMK3) | 595d | 7,806 | |
| 2297 asporulated(pcyt1Ab1) | 215 ± 78 | 1,346 ± 944 | |
| B. thuringiensis SPL 407 (pcyt1Ab1)e | 43.2 ± 21 | 416 ± 219 | |
a
All strains are B. sphaericus, except as noted.
b
LC after 48 h of larval exposure ± standard error; mean of three experiments.
c
Result of two experiments.
d
Result of one experiment.
e
Purified crystals of Cyt1Ab1 protein.