Fig. 6.

Increased oxygen consumption in Thy1-haSyn vs. WT colonic crypts. Oxygen consumption rate (OCR) of freshly isolated colonic crypts was measured by a Seahorse XF Analyzer. (A) OCR traces from n = 3-4 mice per group. Samples sizes and replicate wells per sample are shown in Supplementary Table 5. Error bars represent±SEM. (B) Schematic indicating timing of injections and description of rates in (C). m, months; Oligo, oligomycin; FCCP, carbonilcyanide p-triflouromethoxyphenylhydrazone; AA, antimycinn A; Rot, rotenone, Resp, respiration, Non-Mito, non-mitochondrial respiration; Thy1-haSyn, alpha-synuclein overexpressing; WT, wild type.