Fig. 1.

Characterization of human cortical neurons generated in vitro from pluripotent stem cells. a Schematic of hPSC differentiation into NPCs and mature cortical neurons. The arrows indicate the time point of splitting the cells. b Immunostaining of control and TRPC6 KO cortical neurons (from two different hPSC clones, C21 and C47) differentiated for 8 weeks. Cortical neuron markers; CTIP2, SATB2, TBR2, and BRN2. MAP2, BTUB; pan-neuronal markers. c GFAP, a marker for glia. vGLUT1, a marker for glutamatergic excitatory synapses. d Syn, a synaptic vesicle protein. Nuclei are stained with DAPI. e Validation of TRPC6 protein expression in TRPC6 KO cortical neurons differentiated for 8 weeks. Scale, 100 µm