FIGURE 4.

CTSV is localized in the nuclear compartment of breast cancer cells. (A) Cytoplasmic (C) and nuclear (N) extractions from MCF-7 and ZR75-1 cells identified the presence of CTSV in both cellular compartments by Western blotting. (B) Confocal microscopy was undertaken to confirm the nuclear localisation of CTSV (red) with DAPI nuclear staining (blue). Scale bars = 10 μm (C) Cells sorting and Western blotting analysis suggested that CTSV is localised to the nucleus predominantly during S and G2/M phases. HDAC1 and tubulin expression were included as loading controls relative to the nuclear and cytoplasmic fractions respectively and data presented is representative of at least three independent experiments.