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Figure 4.

Figure 4

Cell apoptosis/necrosis of the IBDV-infected L929 cells stained with annexin V-FITC in conjugation with propidium iodide at different hpi. Each density plot quadrant shows: Q1; necrotic cells (annexin− PI+), Q2; late apoptotic cells (annexin+ PI+), Q3; early apoptotic cells (annexin+ PI−), and Q4; viable cells (annexin− PI−). The graph data show the promotion of early and late apoptosis (Q2 and Q3) in L929 cells. The bar indicates a significant difference (P < 0.05)