Table 2.
Conditions of polymerase chain reaction
| Steps | Temperature (c) | Time | Number of cycle |
|---|---|---|---|
| Initial Denaturation | 95 | 5 minute | 1 |
| Denaturation Final | 95 | 40 second | 35 |
| Annealing | 55 | 40 second | |
| Extention Initial | 72 | 1 minute | |
| Final Extension | 72 | 5 minute | 1 |
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