Table 2. PCR protocols to amplify MS DNA.

Conditions	Initial Denaturation	Denaturation	Annealing	Extension	Final extension
Temperature	94℃	96℃	54℃	68℃	72℃
Time	2 minutes	15 seconds	15 seconds	20 seconds	5 minutes
Cycles	—	35 cycles	35 cycles	35 cycles	—