Fig. 3. The relative mRNA expression of BDNF among the treatment groups showed no significant difference. AA at a 1.45 µg/ml concentration resulted in the highest BDNF expression. Data are presented as the mean ± SEM. The control group showed higher BDNF expression than the hypoxic control group. The higher the concentration of AA given, the higher the expression of BDNF in zebrafish exposed to hypoxia. AA administration at a 1.45 g/ml concentration in zebrafish exposed to hypoxia resulted in high BDNF expression compared with the other groups.