Skip to main content
. 2023 Sep 19;72(12):1766–1780. doi: 10.2337/db23-0289

Figure 2.

Figure 2

Mitochondrial OXPHOS enzyme abundance in offspring skeletal muscle. Protein abundance of electron transport system and OXPHOS complexes was measured with Simple Western for CI (A), CII (B), CIII (C), and CIV (D), CV (E), ETF (F), and ETFDH (G) in offspring soleus (orange bars). Individual protein abundance was normalized to an internal fluorescent loading control, and CI–CV abundance was normalized to total protein. These measures were repeated in the gastroc (blue, I–P). Representative immunoassay images for soleus (H) and gastroc (P) are shown. Dashed horizontal lines indicate different contrast levels. Data were analyzed with two-way ANOVA for significant main effects of m or pw diet or interactions with Sidak multiple comparisons test. P values for significant main effects of m or pw diet are listed above each graph. For post hoc analysis, carets (∧P < 0.05, ∧∧P < 0.01) indicate significant differences by m diet within the same pw diet group and asterisks (*P < 0.05, **P < 0.01) indicate significant differences by pw diet within the same m diet group. Individual data points for offspring on pwCD or pwWD are shown along with group minimum, maximum, and median and interquartile range. M offspring are indicated by circles and F offspring by triangles. Sample size for each group by sex: mCD/pwCD, 6 F/3 M; mCD/pwWD, 2 F/3 M; mWD/pwCD, 3 F/6 M; mWD/pwWD, 5 F/3 M. For ETF and ETFDH n = mCD/pwCD, 4 F/3-4 M; mCD/pwWD, 2 F/3 M; mWD/pwCD, 3 F/3–4 M; mWD/pwWD, 3–5 F/2–3 M.