Table 2.

Enzyme kinetics of WT and mutant GK

Case subject no.	Mutation site	Glucose S0.5 (mmol/L)		ATP Km (mmol/L)	Hill coefficients	Activity index
		No GKA	5 μmol/L GKA
Normal	WT	7.0	2.7	0.32	1.86	1
1, 2	p.E67V	2.8	0.8	0.30	1.72	3
3	p.V91L	1.2	0.5	0.35	1.99	39
4, 5	p.Y215C	2.8	0.8	0.34	1.71	7
6	p.S64P	0.9	1.0	0.38	1.42	10
7	p.S69P	0.6	0.3	0.42	1.31	7
8	p.W99C*	2.4	0.5	0.40	1.35	7
9	p.S69_E70insVPL*	0.6	0.6	0.36	1.31	15
10	p.R447L*	1.1	1.0	0.37	1.29	5
MODY	p.V367M	8.9	2.0	0.35	1.52	0.3

^*Pancreatic islets were isolated and studied.