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. 2023 Apr 20;72(4):439–445. doi: 10.1538/expanim.23-0012

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©2023 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Effects of high fat high cholesterol diet (HFD) on urinary isoprostane (IsoP) (A) and on serum lipoprotein profile (B). Blood and urine samples were collected after 8-weeks of HFD feeding. (A) IsoP in urine collected for 24 h was measured by ELISA as described in Methods. Each column and error bar shows mean and SD, respectively. *: P<0.05 vs. normal diet (MF). No significant difference was observed between SHR^{ApoE(−/−)} and SHR^{ApoE(−/−)Prdx2(−/−)} either under MF or HFD. (B) Serum lipoprotein was fractionated by HPLC, and cholesterol was measured in each fraction as described in Methods. Percent cholesterol against total cholesterol is indicated in the panel. No significant difference in the lipoprotein profile was observed between SHR^{ApoE(−/−)} and SHR^{ApoE(−/−)Prdx2(−/−)}.