FIG. 2.

Amino acid sequence similarities between afc gene products and proteins of known function. (A) Alignment of a portion of AfcA with the amino acid activating domains of several peptide synthetases. Residues similar or identical in at least five are marked #; residues identical or similar in at least three are marked ∗ and +, respectively. I, ATP-binding domain; II, ATPase domain. SafB1, saframycin Mx1 synthetase (U24657); SnbC, pristinamycin synthetase (X98690); Bsu1, polyketide synthetase of Bacillus subtilis (U00024); EntF, enterobactin synthetase component F (P11454); GrsB, gramicidin S synthetase (P14688). (B) Alignment of the predicted amino acid sequences of two portions of AfcC with two segments of the ω-3 fatty acid desaturase (ω-desat) of Brassica napus (L22963). Conserved histidine boxes believed to be associated with the active site are marked (∧∧∧∧∧).