Figure 3.

Photos demonstrating the embedding process for multibrain blocks

(A) A silicone mold is used to ensure the block size will be suitable for standard 25 mm × 75 mm slides.

(B) A printed guide is inserted into the embedding mold, (C) then a glass coverslip with double tape on the top side is placed on top of the printed guide.

(D) The guide is used to assist placement of the brains within the molten wax, the coverslip allows to keep all brains on the same plane, and the double tape keeps the brains in place.

(E) After the wax has set, the silicone mold, printed guide and coverslip with double tape can be easily removed.

(F) Example of a multibrain block with brains positioned for sagittal sections.