Table 2.

Details of the tissue processing times and temperatures for each step

Step	Reagent	Time	Temperature (°C)
Fixation	Perfusion Solution 1	∼30 min	18‒22
	Perfusion Solution 2	∼30 min	18‒22
	Perfusion Solution 2	12 h	4
Storage for MRI	Storage solution	1–27 months	4
Rinsing	dH2O dH2O	<1 min	18‒22
		<1 min	18‒22
Dehydration	70% Ethanol	11 h	18‒22
	80% Ethanol	45 min	18‒22
	95% Ethanol-I	45 min	18‒22
	95% Ethanol-II	45 min	18‒22
	100% Ethanol-I	15 min	18‒22
	100% Ethanol-II	15 min	18‒22
	100% Ethanol-III	15 min	18‒22
	100% Ethanol-IV	30 min	18‒22
	100% Ethanol-V	30 min	18‒22
	100% Ethanol-VI	30 min	18‒22
Clearing	CitroClear-I	30 min	18‒22
	CitroClear-II	45 min	18‒22
	CitroClear-III	60 min	18‒22
Infiltration	Paraffin Wax-I	30 min	60
	Paraffin Wax-II	45 min	60
	Paraffin Wax-III	60 min	60

Note: the storage time is based on how long our samples were stored. Distilled water (dH₂O), minutes (min), hour (h).