Table 3.

Detailed staining conditions for each primary antibody used for mouse FFPE sections

Antibody	Target	Host	Clonality	Antigen retrieval	Working dilution and incubation time
Anti-Brevican (BCAN)	Extracellular matrix, perineuronal nets	Rabbit	Monoclonal	Autoclave, Citrate pH 6	1:2,000, 1 h, 18°C–22°C
Anti-CD68	Macrophages and activated microglia	Rabbit	Polyclonal	Autoclave, Citrate pH 6	1:400, 1 h, 18°C–22°C
Anti-Ferritin FTH1	Iron storage	Rabbit	Monoclonal	Autoclave, Citrate pH 6	1:500, 1 h, 18°C–22°C
Anti-Myelin PLP	Myelin	Rabbit	Polyclonal	Microwave, Citrate pH 6	1:500, 1 h, 18°C–22°C
Anti-NeuN	Mature neurons (nuclei)	Rabbit	Monoclonal	Autoclave, Citrate pH 6	1:3,000, 1 h, 18°C–22°C
Anti-Neurofilament 200 (NF)	Neurofilament heavy polypeptide	Rabbit	Polyclonal	Autoclave, Citrate pH 6	1:2,000, 1 h, 18°C–22°C

Hour (h).