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. 2023 Nov 9;4(4):102681. doi: 10.1016/j.xpro.2023.102681

Table 4.

Comparison of the standard and optimized tissue protocols

Step	Standard			Optimized
Step	Reagent	Time (min)	Temperature (°C)	Reagent	Time (min)	Temperature (°C)
Rinsing	dH₂O	<1	18‒22	dH₂O	<1	18‒22
Rinsing	dH₂O	<1	18‒22	dH₂O	<1	18‒22
Dehydration	70% Ethanol	60	18‒22	70% Ethanol	660	18‒22
	80% Ethanol	60	18‒22	80% Ethanol	45	18‒22
	95% Ethanol-I	60	18‒22	95% Ethanol-I	45	18‒22
	95% Ethanol-II	60	18‒22	95% Ethanol-II	45	18‒22
	100% Ethanol-I	30	18‒22	100% Ethanol-I	15	18‒22
	100% Ethanol-II	30	18‒22	100% Ethanol-II	15	18‒22
	100% Ethanol-III	30	18‒22	100% Ethanol-III	15	18‒22
	100% Ethanol-IV	30	18‒22	100% Ethanol-IV	30	18‒22
	100% Ethanol-V	30	18‒22	100% Ethanol-V	30	18‒22
	100% Ethanol-VI	30	18‒22	100% Ethanol-VI	30	18‒22
Clearing	CitroClear-I	60	18‒22	CitroClear-I	30	18‒22
	CitroClear-II	60	18‒22	CitroClear-II	45	18‒22
	CitroClear-III	60	18‒22	CitroClear-III	60	18‒22
Infiltration	Paraffin Wax-I	60	60	Paraffin Wax-I	30	60
	Paraffin Wax-II	60	60	Paraffin Wax-II	45	60
	Paraffin Wax-III	60	60	Paraffin Wax-III	60	60

Distilled water (dH₂O).