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. 2023 Nov 9;4(4):102681. doi: 10.1016/j.xpro.2023.102681

Tris buffered saline with Triton X (TBS-T) (pH 7.6)

(Washing solution for immunohistochemistry)
Reagent Final concentration Amount
Saline stock 1 x 90 mL
dH2O N/A 810 mL
0.5 M Tris/HCl stock 0.05 M 100 mL
Triton X 0.05% 500 μL
Total N/A 1000 mL