0.5 M Tris/HCl stock (pH 7.4)

(For making TBS-T)
Reagent	Final concentration	Amount
Tris (hydroxymethyl) methylamine	0.5 M	60.5 g
4% HCl	0.8%–1.2%	200–300 mL
dH2O	N/A	500–800 mL
Total	0.5 M	1000 mL