TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant trait(s) | Source or reference |
|---|---|---|
| E. coli | ||
| HB101 | pro leu thi lacY Strr, endoI recA r−, m− | 9 |
| YMC11 | pro thi endA hsr Δ(glnA-glnD) | 2 |
| S. meliloti | ||
| Rm1021 | 2011, Smr | 32 |
| Rm1021-S10 (S10) | 1021::Tn5pRL1063a | This work |
| Rm1021-S11 (S11) | 1021::Tn5pRL1063a | This work |
| Plasmids | ||
| pBSK+ | Multicopy cloning vector | Stratagene |
| pRL1063a | Tn5-luxAB, Smr Kmr OriV | 47 |
| pLAFR1 | Tcr, Cos Tra− Mob+, IncP | 13 |
| pRK2013 | pRK212.2 conjugation helper plasmid | 11 |
| pSIF10 | Circularized 10-kb EcoRI fragment of Rm1021-S10::Tn5-1063 | This work |
| pSIF11 | Circularized 12-kb EcoRI fragment of Rm1021-S11::Tn5-1063 | This work |
| pLES1 | pLAFR1 cosmid of Rm1021 containing stcD | This work |
| pLEB1 | 6.3-kb EcoRI-BamHI fragment carrying stcD in pBSK+ | This work |
| pLBR55 | 6.8-kb EcoRI fragment carrying nodD2 in pBSK+ | 16 |
| pLBR56 | 6.5-kb EcoRI fragment near nodD2 in pBSK+ | 16 |