TABLE 4.
Stachydrine uptake and catabolism by S. meliloti
| Strain | NaCl addeda | Stachydrine uptakeb after:
|
% of 14C incorporatedc
|
||
|---|---|---|---|---|---|
| 10 min | 18 h | Soluble | Insoluble | ||
| Rm1021 | − | 2 | 2 | 54 | 46 |
| + | 18 | 30 | 63 | 37 | |
| Rm1021-S10 | − | 1 | 3 | 87 | 13 |
| + | 16 | 49 | 95 | 5 | |
| Rm1021-S11 | − | 1 | 1 | 61 | 39 |
| + | 20 | 25 | 70 | 30 | |
NaCl added to LAS medium at 300 mM 24 h prior to supplementation with [14C]stachydrine.
Uptake measured as nanomoles per milligram of protein per minute at 10 min and 18 h.
Ethanol-soluble and -insoluble cell fractions determined in the 18-h uptake sample.