Table 2.
Sequences of primers and probes used for comparison of recombination frequency. The TaqMan probes were either labeled by FAM (T. aestivum cv. Chinese Spring [CS] variant) or VIC/HEX (T. militinae [TM] variant). Probes were quenched at their 3′end with QSY or 3IABkFQ with ZEN internal quencher.
| TaqMan probe | Marker | Sequence and modifications 5′–3′ |
|---|---|---|
| owm103_CS_FAM | owm103 | FAM/CTCAAGCTTCAGCGAAGTCGGC/QSY |
| owm103_TM_VIC | VIC/CTCAAGCGTCAGCGAAGTCGG/QSY | |
| owm110_CS_FAM | owm110 | FAM/CAAGTACAC/ZEN/TGAAAATCTAGGCATGTTTC/3IABkFQ |
| owm110_TM_HEX | HEX/CAAGTTCAC/ZEN/TGAAAATCTAGGCATGTTTC/3IABkFQ | |
| owm111_CS_FAM | owm111 | FAM/CTCCCGTGAGCATGCGTCC/QSY |
| owm111_TM_VIC | VIC/TCCCGGACCGTGGCATGC/QSY |