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. 2023 Aug 21;8(6):e10580. doi: 10.1002/btm2.10580

FIGURE 1.

FIGURE 1

In vitro delivery in ALI Calu‐3 cells. (a) Compositions of set 1 LNPs. (b) Quantification of luminescence from ALI Calu‐3 cells 24 h after transfection with aerosolized set 1 LNPs delivering 1000 ng NLuc mRNA. Significance is relative to NLuc F11 (n = 3; mean ± standard deviation [SD], **p < 0.01, ***p < 0.001; Student's t test, double‐tailed). (c) Structures of ionizable lipids used in sets 1 and 2 LNPs. (d) Compositions of set 2 LNPs. (e) Quantification of luminescence from ALI Calu‐3 cells 24 h after transfection with aerosolized set 2 and A‐1 LNPs delivering 1000 ng NLuc mRNA (n = 3; mean ± SD, **p < 0.01, ***p < 0.001; Student's t test, double‐tailed). ALI, air–liquid interface; LNP, lipid nanoparticle.