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. 2023 Aug 21;8(6):e10580. doi: 10.1002/btm2.10580

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© 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of The American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Assessment of cell types in the lungs for in vivo delivery. (a) Schematic of Rosa26 locus in Ai9 mice and delivery schedule of Cre B‐1 LNP. Upon successful Cre‐mediated recombination, the stop cassette will be deleted, and the CAG promoter will drive expression of tdTomato. Aerosolized Cre B‐1 LNP was delivered at 0.5 mg/kg once every other day over a period of 4 days. Lungs were harvested 3 days after the last dose. Created with BioRender.com. (b) Quantification of tdTomato radiance from Ai9 mouse lungs after delivery of Cre B‐1 compared to PBS control. (c) Assessment of tdTomato‐positive cells in endothelial, epithelial, and immune cell populations using flow cytometry (n = 3; mean ± standard deviation, **p < 0.01; Student's t test, double‐tailed). LNP, lipid nanoparticle.