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. 2023 Oct 19;7(11):2214–2221. doi: 10.1021/acsearthspacechem.3c00162

Table 1. Migration Times, Peak Efficiencies, and Resolutions for Analyte Peaks in Neutral and Basic Amino Acid Separation Are Shown in Figure 4aa.

Amino Acid Peak Migration Time (min) Peak Efficiency (plates/m) Resolution (Rs) Peak Area Signal-to-Noise Ratio
Arg 8.95 330000   2.9 × 105 5.2 × 103
Lys 9.13 290000 1.9 4.4 × 105 7.3 × 103
Leu 18.34 310000   3.0 × 105 2.5 × 103
Ile 18.57 320000 1.2 2.7 × 105 2.3 × 103
His/Tyr/Gln 19.00 130000 1.8 4.4 × 105 2.3 × 103
Met 19.35 230000 1.3 3.6 × 105 2.5 × 103
Val 19.82 250000 2.1 2.1 × 105 1.5 × 103
Phe 20.16 260000 1.5 2.0 × 105 1.5 × 103
Asn 20.38 270000 1.0 1.6 × 105 1.1 × 103
Thr/Trp 20.59 200000 0.9 1.8 × 105 1.1 × 103
Pro 21.17 120000 1.9 1.0 × 106 4.6 × 103
Ser 22.58 220000 4.5 2.4 × 105 1.4 × 103
Ala 23.04 230000 1.7 4.3 × 105 2.5 × 103
Gly 26.59 210000 11.8 1.9 × 106 9.0 × 103
a

Resolution for each amino acid peak is calculated with respect to the previously eluted amino acid peak in the standard. Because of dye peaks eluting between Leu and Lys, there is not a resolution calculated between these peaks.