Table 1. Migration Times, Peak Efficiencies, and Resolutions for Analyte Peaks in Neutral and Basic Amino Acid Separation Are Shown in Figure 4aa.
| Amino Acid Peak | Migration Time (min) | Peak Efficiency (plates/m) | Resolution (Rs) | Peak Area | Signal-to-Noise Ratio |
|---|---|---|---|---|---|
| Arg | 8.95 | 330000 | 2.9 × 105 | 5.2 × 103 | |
| Lys | 9.13 | 290000 | 1.9 | 4.4 × 105 | 7.3 × 103 |
| Leu | 18.34 | 310000 | 3.0 × 105 | 2.5 × 103 | |
| Ile | 18.57 | 320000 | 1.2 | 2.7 × 105 | 2.3 × 103 |
| His/Tyr/Gln | 19.00 | 130000 | 1.8 | 4.4 × 105 | 2.3 × 103 |
| Met | 19.35 | 230000 | 1.3 | 3.6 × 105 | 2.5 × 103 |
| Val | 19.82 | 250000 | 2.1 | 2.1 × 105 | 1.5 × 103 |
| Phe | 20.16 | 260000 | 1.5 | 2.0 × 105 | 1.5 × 103 |
| Asn | 20.38 | 270000 | 1.0 | 1.6 × 105 | 1.1 × 103 |
| Thr/Trp | 20.59 | 200000 | 0.9 | 1.8 × 105 | 1.1 × 103 |
| Pro | 21.17 | 120000 | 1.9 | 1.0 × 106 | 4.6 × 103 |
| Ser | 22.58 | 220000 | 4.5 | 2.4 × 105 | 1.4 × 103 |
| Ala | 23.04 | 230000 | 1.7 | 4.3 × 105 | 2.5 × 103 |
| Gly | 26.59 | 210000 | 11.8 | 1.9 × 106 | 9.0 × 103 |
Resolution for each amino acid peak is calculated with respect to the previously eluted amino acid peak in the standard. Because of dye peaks eluting between Leu and Lys, there is not a resolution calculated between these peaks.