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. Author manuscript; available in PMC: 2023 Nov 20.
Published in final edited form as: Biomaterials. 2021 Nov 12;280:121245. doi: 10.1016/j.biomaterials.2021.121245

Fig. 1. Characterization of transport phenomena in a three chambered-microfluidic device.

Fig. 1.

(A) A CAD drawing depicts the overall device design with connecting fluidic lines. (B) Actual size of the device. (C) COMSOL simulation of interstitial flow through the fluidic device predicts flow of ~10 μm/s (D) FRAP experiments in the top (+) and middle (•) of the device (panel C) showed an interstitial flow velocity of 22.58 μm/s and 4.48 μm/s, respectively. Average interstitial flow was 17.7 ± 4.7 μm/s, n = 3. (E) COMSOL modelling of 70 kDa dextran through the right side of the device predicts limited diffusion of dextran through the ports. (F) 70 kDa TRITC-dextran and FITC-dextran perfused through a 10 mg/mL fibrin gel in the device showed limited diffusion between chambers and an even distribution of dextran in the bottom central chamber. (G) Mean fluorescent intensity of an arbitrary line (horizontal white dotted line on the image insets) through the middle ports of the simulated device. Experimentally measured values (blue circles) mapped closely to the theoretical concentration profile (yellow line).