DMI sensitivity of the Δpmr1 mutants. TF01 (ectopic transformant with pUNE10); Δpmr1 mutants DIS07, DIS33, and DIS96; DMI-resistant strain LC2 (parental strain of the transformants); and DMI-sensitive strain PD5 were cultured for 3 days at 25°C on PDA plates containing triflumizole (1 μg/ml), bitertanol (1 μg/ml), fenarimol (1 μg/ml), or pyrifenox (50 μg/ml).