TABLE 3.
Stability of agmatine in 5% BSA under various conditions
| Stability Conditions | Concentration (ng/ml) | % RSD | % Bias | |
|---|---|---|---|---|
| Predicted | Actual | |||
| Room temperature (24 hours) | 8 | 8.57 ± 0.95 | 11.1 | 7.10 |
| 80 | 88.1 ± 2.31 | 2.63 | 10.1 | |
| 800 | 843 ± 20.9 | 2.48 | 5.41 | |
| 4°C (3 days) | 8 | 7.46 ± 0.90 | 12.1 | −6.74 |
| 80 | 84.7 ± 3.28 | 3.88 | 5.92 | |
| 800 | 795 ± 15.5 | 1.95 | −0.65 | |
| Long-term stability (−80°C for 28 days) | 8 | 8.44 ± 0.86 | 10.2 | 5.54 |
| 80 | 79.5 ± 2.00 | 2.52 | −0.68 | |
| 800 | 794 ± 20.2 | 2.64 | −4.48 | |
| Freeze-thaw (3 cycles) | 8 | 7.93 ± 0.54 | 6.82 | −0.87 |
| 80 | 78.2 ± 1.31 | 1.67 | −2.24 | |
| 800 | 781 ± 16.6 | 2.12 | −2.39 | |