Fig. 6.
Lrrc23 knockout causes the downregulation of other flagella component proteins. (A) Left: SDS-PAGE and silver staining were conducted to compare the whole-sperm proteome in Lrrc23+/+ and Lrrc23Δ2/Δ2 mice. Right: mass spectrometry revealed the downregulation of certain flagella components in the spermatozoa of Lrrc23Δ2/Δ2 mice. (B) Western blotting was used to assess flagellum components in protein extracts from spermatozoa. (C) Immunofluorescence staining was conducted for spermatozoa obtained from wild-type and Lrrc23Δ2/Δ2 males using antibodies specific for AC-tub (red) and RSPH9 (green). Nuclei were identified through Hoechst staining (blue). While rectangles indicate regions that have been magnified, with areas of RSPH9 signal discontinuity being marked with white arrowheads.