Fig. 3.
Inhibition of lactate production in MSCs reduces their immunotherapeutic activity in a murine DTH model. A Experimental design of the DTH murine model. B After 24 h post-boost and MSCs injection, paw swelling was measured on the hindlimb paws. C–H After euthanasia, proinflammatory Th1 (C, D) and Th17 (E, F) lymphocytes and anti-inflammatory Treg cells (G, H) present in the draining lymph nodes of DTH mice (black line bars) or DTH mice treated with MSCCTL (gray line bars), MSCGF (orange line bars) or MSCsiLDHA (yellow line bars) were measured by FACS. G Treg/Th17 ratio was also calculated. Each color represents an independent DTH experiment (DTH1: in dark blue; DTH2: in red; DTH3 in yellow). Results represent the mean ± SD of three independent experiments with at least 12 animals per experimental group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Unpaired Anova test). Unless otherwise indicated, comparisons were with untreated DTH mice