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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: Fertil Steril. 2023 Jul 31;120(5):1061–1070. doi: 10.1016/j.fertnstert.2023.07.024

Figure 4.

Figure 4.

TOP5300 stimulates greater expression of STAR and CYP19A1 than rh-FSH following 6 hrs of culture and stimulates more robust response among PCOS patients compared to NOR or ARA patients. Steroid enzyme expression (determined by qPCR; see Materials & Methods) for basal, rh-FSH and TOP5300 treated cells were analyzed (ANOVA) for each compound and comparisons were made among patient diagnoses and FSHR agonist treatments. Expression of STAR (A) and CYP19A1 (B) in GLC was found to be significantly higher in TOP5300 treated cells than rh-FSH-treated cells (n = 6). (c) Integrated expression of StAR and CYP19A1 was significantly higher in TOP5300 treated cells compared to rh-FSH-treated cells for PCOS patients, while rh-FSH was similar to basal levels (n = 6). (d) Integrated expression of StAR and CYP19A1 was stimulated significantly above basal by both TOP5300 and rh-FSH, while the difference between the two ligands was not different in cells from NOR or ARA patients (n = 6). Mixed effects, 2-way ANOVA.