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. 2023 Nov 6;11:1296132. doi: 10.3389/fbioe.2023.1296132

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This work is authored by Jinho Kim and Taek Soon Lee on behalf of the U.S. Government and as regards Dr. Kim, Dr. Lee and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Growth and (B) isoprenol production by strains harboring single gRNAs and the original MVA pathway. Batch cultures were grown in 5 mL M9-MOPS minimal medium supplemented with 20 g/L of glucose (n = 3). (C) Scatter plots of growth rate and isoprenol titer at 48 h and (D) 72 h show that selected gRNA targets were leveraged for high isoprenol production while maintaining a high growth rate relative to the control. The control harboring non-targeting gRNA plasmid JBEI-18706 is demarcated with a bright yellow triangle.