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. 2023 Nov 6;11:1296132. doi: 10.3389/fbioe.2023.1296132

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This work is authored by Jinho Kim and Taek Soon Lee on behalf of the U.S. Government and as regards Dr. Kim, Dr. Lee and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Isoprenol production and (B) growth of strains harboring single gRNAs and the IPP-bypass pathway. Cultures were grown in 5 mL M9-MOPS minimal medium supplemented with 20 g/L of glucose (n = 3). (C) Scatter plots of growth rate and isoprenol titer at 48 h and (D) 72 h show that gene attenuation by certain gRNAs contributed to significant titer improvements, tracking well with observations from the original MVA pathway experiments. The control is demarcated with a yellow triangle.