TABLE 1.

List of base strains and plasmids used in this study.

Strains	Description	Reference
Isoprenol pathway strains
E. coli DH1	F− supE44 hsdR17 recA1 gyrA96 relA1 endA1 thi−1 lambda-	Wild type
OriMVA	E. coli DH1 harboring JBEI-6829 and JPUB_004507	George et al. (2018)
IPP-By	E. coli DH1 harboring JBEI-9310 and JBEI-14559	Kang et al. (2019)

Plasmids	Description	Reference
Isoprenol pathway plasmids
OriMVA-Upper	pLacUV5-atoB-HMGS_Sa-HMGR_Sa-MKco-PMKco, p15a, CmR	George et al. (2018)
OriMVA-Lower	pTrc99A-NudBR10-PMD_Sc, pBR322, AmR	George et al. (2018)
IPPBy-Upper	pLacUV5-atoB-HMGS_Sa-HMGR_Sa, p15a, CmR	Kang et al. (2019)
IPPBy-Lower	pTrc-PMD_R74H-R147K-M212Q, pBR322, AmR	Kang et al. (2019)
Single gRNA plasmids (pSC101 origin, KanR, dCas9 under pTet promoter, gRNA under pJ23119)
Non-target Control (JBEI-18706)	pTet-dCas9-pJ23119-NT_Control, pSC101, KanR	Tian et al. (2019)
accA	pTet-dCas9-pJ23119-accA, pSC101, KanR	Tian et al. (2019)
ackA	pTet-dCas9-pJ23119-ackA, pSC101, KanR	Tian et al. (2019)
adhE	pTet-dCas9-pJ23119-adhE, pSC101, KanR	This study
arcC	pTet-dCas9-pJ23119-arcC, pSC101, KanR	Tian et al. (2019)
asnA	pTet-dCas9-pJ23119-asnA, pSC101, KanR	Tian et al. (2019)
citE	pTet-dCas9-pJ23119-citE, pSC101, KanR	This study
dacA	pTet-dCas9-pJ23119-dacA, pSC101, KanR	Tian et al. (2019)
deoB	pTet-dCas9-pJ23119-deoB, pSC101, KanR	Tian et al. (2019)
eutD	pTet-dCas9-pJ23119-eutD, pSC101, KanR	Tian et al. (2019)
fabH	pTet-dCas9-pJ23119-fabH, pSC101, KanR	This study
gldA	pTet-dCas9-pJ23119-gldA, pSC101, KanR	Tian et al. (2019)
ispA	pTet-dCas9-pJ23119-ispA, pSC101, KanR	Tian et al. (2019)
ldhA	pTet-dCas9-pJ23119-ldhA, pSC101, KanR	This study
mdh	pTet-dCas9-pJ23119-mdh, pSC101, KanR	Tian et al. (2019)
menA	pTet-dCas9-pJ23119-menA, pSC101, KanR	This study
mqo	pTet-dCas9-pJ23119-mqo, pSC101, KanR	Tian et al. (2019)
pgl	pTet-dCas9-pJ23119-pgl, pSC101, KanR	This study
poxB	pTet-dCas9-pJ23119-poxB, pSC101, KanR	This study
ppc	pTet-dCas9-pJ23119-ppc, pSC101, KanR	Tian et al. (2019)
ppsA	pTet-dCas9-pJ23119-ppsA, pSC101, KanR	This study
prpE	pTet-dCas9-pJ23119-prpE, pSC101, KanR	Tian et al. (2019)
pta	pTet-dCas9-pJ23119-pta, pSC101, KanR	Tian et al. (2019)
rssA	pTet-dCas9-pJ23119-rssA, pSC101, KanR	This study
sdhABCD	pTet-dCas9-pJ23119-sdhABCD, pSC101, KanR	This study
sucA	pTet-dCas9-pJ23119-sucA, pSC101, KanR	This study
sucB	pTet-dCas9-pJ23119-sucB, pSC101, KanR	This study
sucCD	pTet-dCas9-pJ23119-sucCD, pSC101, KanR	This study
thrC	pTet-dCas9-pJ23119-thrC, pSC101, KanR	Tian et al. (2019)
ubiA	pTet-dCas9-pJ23119-ubiA, pSC101, KanR	This study
yahl	pTet-dCas9-pJ23119-yahl, pSC101, KanR	Tian et al. (2019)
yhfW	pTet-dCas9-pJ23119-yhfW, pSC101, KanR	Tian et al. (2019)
yqeA	pTet-dCas9-pJ23119-yqeA, pSC101, KanR	Tian et al. (2019)
Double gRNA plasmids (pSC101 origin, KanR, dCas9 under pTet promoter, gRNA’s under pJ23119)
fabH-ubiA	pTet-dCas9-pJ23119-fabH-ubiA, pSC101, KanR	This study
adhE-menA	pTet-dCas9-pJ23119-adhE-menA, pSC101, KanR	This study
ldlhA-ubiA	pTet-dCas9-pJ23119-ldhA-ubiA, pSC101, KanR	This study
ldhA-fabH	pTet-dCas9-pJ23119-ldhA-fabH, pSC101, KanR	This study
ldhA-menA	pTet-dCas9-pJ23119-ldhA-menA, pSC101, KanR	This study
adhE-fabH	pTet-dCas9-pJ23119-adhE-fabH, pSC101, KanR	This study
ldhA-adhE	pTet-dCas9-pJ23119-ldhA-adhE, pSC101, KanR	This study
adhE-ubiA	pTet-dCas9-pJ23119-adhE-ubiA, pSC101, KanR	This study
fabH-menA	pTet-dCas9-pJ23119-fabH-menA, pSC101, KanR	This study
menA-ubiA	pTet-dCas9-pJ23119-menA-ubiA, pSC101, KanR	This study
ppsA-adhE	pTet-dCas9-pJ23119-ppsA-adhE, pSC101, KanR	This study
ppsA-fabH	pTet-dCas9-pJ23119-ppsA-fabH, pSC101, KanR	This study
ppsA-ldhA	pTet-dCas9-pJ23119-ppsA-ldhA, pSC101, KanR	This study
ppsA-menA	pTet-dCas9-pJ23119-ppsA-menA, pSC101, KanR	This study
ppsA-ubiA	pTet-dCas9-pJ23119-ppsA-ubiA, pSC101, KanR	This study
Triple gRNA plasmids
adhE-fabH-ldhA	pTet-dCas9-pJ23119-adhE-fabH-ldhA, pSC101, KanR	This study
adhE-fabH-menA	pTet-dCas9-pJ23119-adhE-fabH-ldhA, pSC101, KanR	This study
adhE-fabH-ubiA	pTet-dCas9-pJ23119-adhE-fabH-ldhA, pSC101, KanR	This study
menA-ubiA-fabH	pTet-dCas9-pJ23119-menA-ubiA-fabH, pSC101, KanR	This study