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. 2023 Nov 8;19(11):e1011585. doi: 10.1371/journal.ppat.1011585

Fig 3. Live imaging of iRBC incubated with NK cells.

Fig 3

(A, B) eFluor 450-stained primary NK cells (blue) incubated with eFluor 670-stained iRBC (red) at a ratio of 3:1 in the presence of 2 μg/ml anti-RBC IgG. PI was added at the start of image recording. The number in each panel indicates the time in minutes relative to the first NK–iRBC contact (set as minute 0). The effector NK cell is marked with an asterisk and the iRBC target with a T in the first frame. The first appearance of a PI-positive parasite is marked with a yellow arrow. Six time frames spanning 11 minutes are shown in (A) and eight timeframes spanning 159 minutes are shown in (B). The scale bar represents 10 μM.